Propagation of Ammi visnaga (L.) Under the Influence of Inorganic of Some Chromium Metabolites Khellin, Visnaginby in Vitro Culture Technique

Abstract

The experiment was carried out. To know the effect of adding KNO₃, KH₂PO₄, and CaCl₂ to the callus of the plant Ammi visnaga that produces metabolic compounds khellin and visnagin. The seeds were planted on Woody plant medium (WPM) supplemented with 2,4-Dichlorophenoxyacetic acid at levels 0, 1.5, 2.0, 2.5 and 3.0 mg L^-1 mixed with Kinetin 6-furfurylo- amino-purine a level of 2.5 mg L^-1. After germination of the seeds the shoot tips were taken and grown on WPM medium supplemented with 2,4-D at 0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0 and 5.5 mg L^-1 mixed with kinetin at a level. 2.5 mg L^-1. After obtaining the callus, it was grown on WPM medium supplied with KNO₃ at a level of 0.0, 0.20, 0.30 and 0.40 g L^-1 and KH₂PO₄ at a level of 0.0, 0.20, 0.25 and 0.30 g L^-1 and CaCl₂.2H₂O at a level of 0.0, 0.20, 0.30 and 0.40 g L^-1.The results showed that the level was 3.0 mg L^-1 of 2,4-D and Kinetin at 2.5 mg L^-1 gave the highest germination rate. The level of 3.0 mg L^-1 2,4-D with 2.5 mg L^-1 Kinetin achieved the highest percentage of callus formation 95.80%. The highest level of visnagin and khellin was obtained when adding KNO₃ at a level of 0.30 g L^-1, KH₂PO₄ at a level of 0.30 g L^-1 and CaCl₂ at a level of 0.20 g L^-1. The addition of element salts affected the callus content of khellin and vinsangen compounds.