EFFECT OF PLANT GROWTH REGULATORS AND TYPE OF EXPLANT ON PHENOLIC PRODUCTION FROM STRAWBERRY PLANT IN VIVO AND IN VITRO

Abstract

This experiment was conducted in the plastic house and the Lath house /College of Agriculture/ Diyala University while the tissue experiment was conducted in the plant tissue culture lab./ Horticulture Dept./ College of Agriculture/ Diyala University. The aim of this research is to study the effect of plant growth regulators on initiation of callus and phenolic compound in callus and comparing them with the plants in the field. The effect of the interaction of 2,4D with Kin. or BA. The result showed when the medium supplemented with 1 mg l-1 Kin. + 1 or 2 mg l-1 2,4-D increase the fresh and dry weight of callus to 2.560 and 0.146 g respectively. The results of the effect of interaction NAA with BA or Kin. The result showed when the medium supplemented with 1.5 mg l-1 Kin. + 1 mg l-1 NAA increase the fresh and dry weight of callus to 2.107 g and 0.127 g respectively. On the experiment of evaluating phenolices from the callus and the planted tissues in the field, the results showed that the particular tissues gave the high levels of phenolices compounds as compared to callus tissues. The leaf of Fragaria vesca gave high quantity of merycitn compounds and caffic acid and gallic acid. The flowers of the same type gave the quantity of alpha penine compounds, comarine and quercetin. The roots of the same type gave high quantity of P-hydroxy benzoic acid and ferulic acid. The callus of the same type gave the high quantity of camphene compound and ellagic acid, while Salwan type gave high quantity of catachin compound.