EFFECT OF FOLIAR APPLICATION AND SOAKING OF SEED WITH H2O2 ON SAME ENZYMATIC ANTIOXIDANT Vigna radiata L.

Abstract

Two field experimentswere conducted during spring and autumn seasons of Vigna radiata L.in the year 2014  at botanical garden of Department of  Biology ،College of Education for Pure Sciences Ibn-Al-Haithem ، University of Baghdad .The experiments aimed to study the effect of different concentrations of  glutathione (25 ، 50 ،75،100 mg.L-1 ) added to control treatment  and soaking of seed with H2O2 5،10،15 mM.L-1 on some enzymatic antioxidant of Vigna radiata L. . The results showed an increase in total activity of Superoxide dismutase (SOD) with 100 mg.L-1 of glutathione by 69.32% ، 40.93% for the two seasons and increased with 15 mM.L-1 H2O2 by 100% ، 28.53% . The interaction between the two treatments was significant .The activity of Peroxidase (POD) was increased with 100mg.L-1 glutathione by 29.45% ، 88.15% and with 15 mM.L-1 of H2O2 175.57% ، 40.58%،also the interaction between the two treatment was significant .The total activity of Catalase  (CAT) increased with 100 mg.L-1 of glutathione by 55.47% for first season only and increased with 15mM.L-1 of H2O2 by 118.29% ، 71.78% .The interaction was significant between the two factors of experiment .The result showed that the total  activity of glutathione peroxidase(GPX)increased with 100 mg.L-1 of glutathione by 30.905 ، 63.62% also increased with 15mM.L-1 of H2O2 by 12.86% ، 61.40% for two seasons ، the interaction  between  the two treatment was significant for the first growth season. All results compared with control plants. The result showed the concentration of 100 mg.L-1 glutathione and concentration of 15 mM.L-1 hydrogen peroxide caused an increased for most study parameters.