DIAGNOSTIC OF WIREWORM THAT ATTAK POTATO TUBERS BY USING PCR

Abstract

 Field studies were carried out in the middle of Iraq during 2009  -  2012 to detect and identify the species of genus Agriotes spp that infest Potato tubers (Solanum tubersum  L. ) by using Molecular diagnosis Polymerase Chain Reaction (PCR) for both adults and larvae . The results showed that there are five species of Agriotes infest Potato tubers in Spring and Autumn cultivation . These species are :  Agriotes brevis  (Candeze) ,  Agriotes lineatus(Linnaeus) Agriotes obscurus (Linnaeus) , Agriotes sputator(Linnaeus) Agriotes ustulatus (Schaller). A genetic diversity within the species of the genus Agriotes was found