STUDY THE ANALGESIC AND SEDATIVE EFFECT OF Ocimum basilicum ALCOHOLIC EXTRACT IN MALE RATS

Abstract

This study was carried out on male rats to evaluate the analgesic and sedative effect of different doses of Alcoholic Extract of Ocimum basilicum leaves. The analgesic effect studied by using formalin test, 20 male rats divided to a four groups (5 each), first group exposed water orally only before injection of formalin, second group exposed orally with Diclofenac at a dose 0.71mg/kg B.W before injection of formalin, Third and fourth groups orally incubated with Alcoholic Extract of Ocimum basilicum leaves at a dose (50 and 100mg/kg BW) respectively. While the sedative effect studied by using pentobarbitone sleeping time test and open field test, in each test 15 male rats used and divided to a three groups one of them treated with distillated water and the other two groups treated with Alcoholic Extract of Ocimum basilicum leaves at a dose (50 and 100mg/kg BW) respectively. Results of formalin test showed a significant reduction in the mean value of nociceptive response in Diclofenac group specially at late phase while, groups treated with Alcoholic Extract of Ocimum basilicum leaves revealed a significant reduction in the nociceptive response value at the both phases (early and late phase) moreover the group treated with 100mg/kg showed the highest attenuation in the mean value of nociceptive response compared to 50mg/ kg treated group. Furthermore, the dose 100mg/kg produced the potent sedative effect in pentobarbitone sleeping time test and open field test. These results pointed to analgesic and sedative effect of Ocimum basilicum may duo to its consistence of the active analgesic and sedative compounds and its effect are in a dose dependent manner