EFFECT OF THE ANTIBIOTICS ON GLUTAMINASE PURIFIED FROM Serratia plymuthica ISOLATED FROM DIYALA RIVE

Abstract

Serratia plymuthica isolates were obtained at 20 isolates out of 40 water samples from Diyala river, Baquba city ,these isolates were tested for sensitivity
against 10 different antibiotics .The results showed that the isolates have littlie
resistance to Nitrofurantion(N),Chloramphenicol(C) and Streptomycin(S ), but they were giving whole activity (100%) toward Amoxicillin (AMX), Ampiclocx,(AM ) and Cephalothin(CF) antibiotics. Active of these isolates was selected to purify glutaminase through several stages of purification including (ammonium sulfate precipitation ,ion exchange by DEAE- cellulose, and gel filtration by sephadex G-100). The stock solution of these antibiotics was mixed with purified enzyme respectively The results showed that the activity was reduced to zero by using the ,(AM ) , Metronidazole, (CF) ,Erythromycin, and Rifampicin . On the other hand the Tetracycline and (C) were raised the specific activity of glutaminase to 10.8 I.U./mg and 15.31 U.I./mg respectively
in the comparison with the control ( 3.1 I.U./mg ).