WATERY EXTRACT AND METHONOLIC EXTRACTION OF NERIUM OLEANDER EFFECT ON MICROTUBULES OFH22 CELL AND PROGRAMMED CELL DEATH

Abstract

The study evaluated the effect of selected Oleandrin and methonolic extraction of Nerium oleander on the microtubule network of H22 cells line (hepatic cell) GFP tubulin labeled . Cells were treated with watery extract and methonolic extraction at various concentration from 0.01 µ/ml to 10m/ml for 60 min , , microtubules were detected by mean of indirect immunfluorescence. the damage was examined in afluorescnce microscope . Therapeutic effect of both extracts was studied in tumor-bearing mice after (I.P) administration of watery extract at concentrations of methonolic (16.5 , 8.1, 4.1mg/kg) and methonoloic extract at concentrations (20, 10, 5 mg/kg) for 30 days alternatively. The results revealed significant reduction in the tumor volume weather in those treated with watery extract 84.09%, or those treated with alcoholic extract 86.07%, particularly at the concentration of 16.5 mg/kg and 20 mg/kg respectively .