THE EFFECT OF DENSITIES CULTURE OF CELL SUSPENSION PLANT Withania somnifera L. IN CELL PLATING AND ARRAY METHOD IN THE FORMATION OF CALLUS PRIMORDIAS
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Abstract
This study succeeded in establish cell suspension derived from callus hypocotyle
Withania somnifera L. seedlings, induced in MS solid medium supplemented with
3.0 mg l
-1 2,4-D Intertwined with 0.5 mg.L-1 Kin. Detection culture different
densities (1.0, 1.52, 1.79, 2.03) ×104
cell cm-3 of these suspensions of plating and
array method of agar-solidified MS medium in the presence of concentrations 3.0
mg l
-1 2,4-D + 0.5 mg l
-1 Kin. Culture method by plating was exceeding the array
method in the number of cellular colonies. It has reached the 27.600 colony dish-1
at the culture density 2.03 ×104
cell cm-3
compared with the initiation density
which amounted to 8.125 colony dish-1
in plating method, while the number of
these colonies by array method was 21.400 colony dish-1 using the same culture
density compared with the initiation density which amounted to 10.400 colony
dish-1
. Moral high density excelled significantelly on the rest of the densities opals
giving 58 callus primordial after 20 days of culture of cells suspension using
plating method, and giving 6 callus primordial after 25 days of culturing on cell
suspension when array method was used.