Effectiveness of Essential Oils from Three Medicinal Plants Against Bayoud Disease (Fusarium oxysporum f. sp. albedinis) of Date Palm (Phoenix dactylifera L.)

Abstract

Plant extracts and essential oils are promising new sources of non-toxic alternatives to chemicals substances. They are used for their antimicrobial properties against plant diseases of fungal origin, against bacterial, and those of virus origin. This study was carried out to study the antifungal activity of Artemisia herba-alba, Foeniculum vulgare and Citrus sinensis essential oils against Fusarium oxysporum f. sp. albedinis. Essential oil was extracted by steam distillation. Antifungal activity of essential oil was investigated by macro-broth method of dilution by a minimal inhibitory concentration (MIC) assay against this pathogen. The yield of essential oil obtained by steam distillation of Foeniculum vulgare samples was 2.31% greater than that obtained from Citrus sinensis sample which was 1.8%, followed by Artemisia herba alba samples 1.22%. Regarding antifungal activity, the results revealed a better inhibitory activity of Artemisia herba alba against the tested strainsat the lowest LC₅₀ values (0.1 µl/ml). On the other hand, Foeniculum vulgare, Artemisia herba alba and Citrus sinensis essential oils show similar MICs of mycelial growth against this pathogen. The value of the MIC and CMF is greater than 50 µl/ml for the three essential oils