Study of HSP70 Gene and Relationship to Milk Productivity and Fat Ratio Holstein Cows

Abstract

The study was conducted on 75 Holstein cows imported from Germany to determine the chi-square value, the breeding value, the additive and dominance effect, and the effect of the allele of the HSP70 gene on the variation of total milk production and fat ratio. The HSP70 gene was divided into three bands, each with a length of 922, 935 and 932 bp. Molecular analysis of the gene shows the presence of 12-point mutations distributed in three primers (T825C, G856A, G937A, A1135G, G1185C, G1753A, C1867T, A2017G, C2548T, G2572T, G2688C and T2734C). Mutation T825C changed the amino acid Ata to Lie, and mutation G856A changed the amino acid Aca. to Thr. The result showed that the chi-square value was significant in the mutations (C1867T, A2017G and G2688C) reached (4.25, 3.90 and 4.16). The higher milk production in the CT genotype of the C1867T mutation was 5442.7 kg/season, the higher breeding value, dominance deviation, additive and dominance variance (652.3, 1460, 726856 and 235183) respectively for total milk production. The higher ratio of fat in the CT genotype of the C2548T mutation was (5.04%), and the higher breeding value, dominance deviation, additive and dominance variance (1.30, 1.70, 0.576 and 0.0793), respectively for the ratio of fat.